Browsing by Author "Josephine, Kasolo"

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    Low concentrations of Lactobacillus rhamnosus GG ( Yoba®) are safe in male Drosophila melanogaster
    (Research Note, 2019) Keneth Iceland, Kasozi; Aisha, Bukenya; Ejike, Daniel Eze; Josephine, Kasolo; Dickson Stuart, Tayebwa; Fred, Ssempijja; Joy, Suubo; Andrew, Tamale; Isaac, Echoru; Ibrahim, Ntulume; Sarah Kemuma, Onkoba; Lisa Nkatha, Micheni; Emmanuel Tiyo, Ayikobua; Oscar Hilary, Asiimwe; Muhamudu, Kalange
    Objective: The purpose of the study was to generate information on the safety of probiotics, thus the study objectives were to evaluate the effects of Yoba ® on basic physiochemical properties. The study assessed male w1118 Drosophila melanogaster which were provided food supplemented with Yoba ® at 1%, 3%, 6%, and 12% on motor function, total protein, catalase activity, and hydrogen peroxide scavenging activity and lifespan. Results: Yoba® at high concentration (≥ 6%) increased locomotor activity in Drosophila melanogaster, however, total protein, catalase, and hydrogen peroxide scavenging activity were significantly higher at 1% Yoba ® compared to 3%, 6%, and 12% Yoba ®. Yoba consumed at 1% was associated with greater physiological benefits in Drosophila melanogaster. Findings in the study offer a rationale for the consumption of Yoba ® at 1% in humans as is currently being promoted by the Yoba for Life consortium, however, high concentrations of Yoba ® would disrupt physiological function as shown by this study.
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    ItemOpen Access
    Low concentrations of Lactobacillus rhamnosus GG ( Yoba®) are safe in male Drosophila melanogaster
    (BMC Res Notes, 2019) Keneth Iceland, Kasozi; Aisha, Bukenya; Ejike, Daniel Eze; Josephine, Kasolo; Dickson Stuart, Tayebwa; Fred, Ssempijja; Joy, Suubo; Andrew, Tamale; Isaac, Echoru; Ibrahim, Ntulume; Sarah, Kemuma Onkoba; Lisa Nkatha, Micheni; Emmanuel Tiyo, Ayikobua; Oscar Hilary, Asiimwe; Muhamudu, Kalange
    Objective: The purpose of the study was to generate information on the safety of probiotics, thus the study objectives were to evaluate the effects of Yoba ® on basic physiochemical properties. The study assessed male w1118 Drosophila melanogaster which were provided food supplemented with Yoba ® at 1%, 3%, 6%, and 12% on motor function, total protein, catalase activity, and hydrogen peroxide scavenging activity and lifespan. Results: Yoba® at high concentration (≥ 6%) increased locomotor activity in Drosophila melanogaster, however, total protein, catalase, and hydrogen peroxide scavenging activity were significantly higher at 1% Yoba ® compared to 3%, 6%, and 12% Yoba ®. Yoba consumed at 1% was associated with greater physiological benefits in Drosophila melanogaster. Findings in the study offer a rationale for the consumption of Yoba ® at 1% in humans as is currently being promoted by the Yoba for Life consortium, however, high concentrations of Yoba ® would disrupt physiological function as shown by this study.
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    Lycopene improves on basic hematological and immunological parameters in diabetes mellitus
    (BMC Research Notes, 2019) Ejike, Daniel Eze; Adam, Moyosore Afodun; Josephine, Kasolo; Keneth Iceland, Kasozi
    Objective: Diabetes is associated with an upset of hematological and immunological parameters in humans, how- ever information on the effects of Lycopene is scarce. The aim of the study was to gain information on basic changes in hematological parameters as markers for safety since anemia as a complication in diabetic chemotherapy has been reported. Results: Lycopene had anti-anemic effects and improved on the immune status of diabetic rats and these obser- vations were dose independent. There was a decrease in neutrophil, low neutrophil–lymphocyte ratio and platelet counts and stable albumin, globulin levels. Lycopene could exert its protective effects through a balance of basic hematological physiological variables.
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    Lycopene improves on basic hematological and immunological parameters in diabetes mellitus
    (BMC, 2019) Ejike, Daniel Eze; Adam Moyosore, Afodun; Josephine, Kasolo; Keneth Iceland, Kasozi
    Objective: Diabetes is associated with an upset of hematological and immunological parameters in humans, however information on the effects of Lycopene is scarce. The aim of the study was to gain information on basic changes in hematological parameters as markers for safety since anemia as a complication in diabetic chemotherapy has been reported. Results: Lycopene had anti-anemic effects and improved on the immune status of diabetic rats and these observations were dose independent. There was a decrease in neutrophil, low neutrophil–lymphocyte ratio and platelet counts and stable albumin, globulin levels. Lycopene could exert its protective effects through a balance of basic hematological physiological variables.
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    Synergistic action of propolis with levodopa in the management of Parkinsonism in Drosophila melanogaster
    (Journal of Complementary and Integrative Medicine, 2020) Herbert Izo, Ninsiima; Emmanuel Tiyo, Ayikobua; Josephine, Kasolo; Keneth Iceland, Kasozi; Ejike, Daniel Eze
    Background: The Phosphatase and tensin-induced putative kinase 1 (PINK1B9) mutant for Drosophila melanogaster is a key tool that has been used in assessing the pathology of Parkinsonism and its possible remedy. This research was targeted toward determining the effects of ethanolic extract of propolis, with levodopa therapy in the management of Parkinsonism. Method: The PINK1B9 flies were divided into groups and fed with the different treatment doses of ethanoic extract of propolis. The treatment groups were subjected to 21 days of administration of propolis and the levodopa at different doses after which percentage climbing index, antioxidant activity and lifespan studies were done. Results: Propolis alone improved motor activity, antioxidant and lifespan in Drosophila melanogaster than in PINK1 flies. Propolis in combination with levodopa significantly (P<0.05) improved physiological parameters at higher than lower concentrations in Parkinsonism Drosophila melanogaster demonstrating its importance in managing side effects associated with levodopa. Conclusion: Propolis is a novel candidate as an alternative and integrative medicinal option to use in the management of Parkinsonism in both animals and humans at higher concentrations.